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arac p bad 1286 bp fragment  (Addgene inc)


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    Addgene inc arac p bad 1286 bp fragment
    Arac P Bad 1286 Bp Fragment, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/arac+p+bad+1286+bp+fragment/pJeM1+(Plasmid+%23135088)/pmc11983282-63-19-27
    Average 93 stars, based on 4 article reviews
    arac p bad 1286 bp fragment - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Nucleotide-level characterization and improvement of l -arabinose- and l -rhamnose-inducible systems in E. coli using a high-throughput approach
    Article Snippet: .. The pNK93 plasmid was first constructed by amplifying the vector backbone (3176 bp) of pNK25 plasmid [ ] and AraC-P BAD (1286 bp) fragment from pJeM1 plasmid (Addgene, cat. #135088) were amplified with Q5 High-Fidelity 2X Master Mix. .. Once fragment sizes were verified on 0.8% agarose (VWR, cat. #0710-500G), polymerase chain reaction (PCR) products were purified, digested with DpnI to remove the parental template, and then assembled using NEBuilder HiFi DNA Assembly Master Mix.

    Construct:

    Article Title: Nucleotide-level characterization and improvement of l -arabinose- and l -rhamnose-inducible systems in E. coli using a high-throughput approach
    Article Snippet: .. The pNK93 plasmid was first constructed by amplifying the vector backbone (3176 bp) of pNK25 plasmid [ ] and AraC-P BAD (1286 bp) fragment from pJeM1 plasmid (Addgene, cat. #135088) were amplified with Q5 High-Fidelity 2X Master Mix. .. Once fragment sizes were verified on 0.8% agarose (VWR, cat. #0710-500G), polymerase chain reaction (PCR) products were purified, digested with DpnI to remove the parental template, and then assembled using NEBuilder HiFi DNA Assembly Master Mix.

    Amplification:

    Article Title: Nucleotide-level characterization and improvement of l -arabinose- and l -rhamnose-inducible systems in E. coli using a high-throughput approach
    Article Snippet: .. The pNK93 plasmid was first constructed by amplifying the vector backbone (3176 bp) of pNK25 plasmid [ ] and AraC-P BAD (1286 bp) fragment from pJeM1 plasmid (Addgene, cat. #135088) were amplified with Q5 High-Fidelity 2X Master Mix. .. Once fragment sizes were verified on 0.8% agarose (VWR, cat. #0710-500G), polymerase chain reaction (PCR) products were purified, digested with DpnI to remove the parental template, and then assembled using NEBuilder HiFi DNA Assembly Master Mix.



    Similar Products

    arac p bad 1286 bp fragment  (Addgene inc)
    93
    Addgene inc arac p bad 1286 bp fragment
    Arac P Bad 1286 Bp Fragment, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/arac+p+bad+1286+bp+fragment/pJeM1+(Plasmid+%23135088)/pmc11983282-63-19-27
    Average 93 stars, based on 1 article reviews
    arac p bad 1286 bp fragment - by Bioz Stars, 2026-11
    93/100 stars
    		  Buy from Supplier

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